KIAA0513 Polyklonaler Antikörper

KIAA0513 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21478-1-PBS

Synonyme

Uncharacterized protein KIAA0513

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  WB analysis of mouse brain using 21478-1-AP (same clone as 21478-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21478-1-AP (KIAA0513 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21478-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 21478-1-AP (same clone as 21478-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21478-1-AP (KIAA0513 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21478-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 21478-1-AP (same clone as 21478-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 21478-1-AP (KIAA0513 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21478-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 21478-1-AP (same clone as 21478-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 21478-1-AP (KIAA0513 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21478-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse cerebellum using 21478-1-AP (same clone as 21478-1-PBS)

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 21478-1-AP (KIAA0513 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21478-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 21478-1-AP (same clone as 21478-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 21478-1-AP (KIAA0513 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21478-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 21478-1-AP (same clone as 21478-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 21478-1-AP (KIAA0513 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21478-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 21478-1-AP (same clone as 21478-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using KIAA0513 antibody 21478-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 21478-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

21478-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA KIAA0513 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KIAA0513 fusion protein Ag15624
• Vollständiger Name: KIAA0513
• Berechnetes Molekulargewicht: 411 aa, 47 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC030280
• Gene symbol: KIAA0513
• Gene ID (NCBI): 9764
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KIAA0513 was identified as upregulated in subjects with schizophrenia. The mRNA of KIAA0513 is ubiquitously expressed but is enriched in the brain, particularly in the cerebellum. The interacting protein analysis indicated that KIAA0513 is involved in both synaptic and apoptotic signaling. (PMID: 17010949) Several isoforms of KIAA0513 (47/46/34 kDa) exist due to the alternative splicing. This antibody is expected to recognize all of isoforms of KIAA0513.