KIAA0528 Polyklonaler Antikörper

KIAA0528 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21138-1-PBS

Synonyme

CDP138, C2CD5, C2 domain-containing protein 5, C2 domain-containing phosphoprotein of 138 kDa

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  WB analysis of HeLa using 21138-1-AP (same clone as 21138-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 21138-1-AP (KIAA0528 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21138-1-PBS in a different storage buffer formulation.

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  WB analysis using 21138-1-AP (same clone as 21138-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21138-1-AP (KIAA0528 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21138-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 21138-1-AP (same clone as 21138-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 21138-1-AP (KIAA0528 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21138-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 21138-1-AP (same clone as 21138-1-PBS)

  IP result of anti-KIAA0528 (IP:21138-1-AP, 4ug; Detection:21138-1-AP 1:700) with HeLa cells lysate 520ug. This data was developed using the same antibody clone with 21138-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 21138-1-AP (same clone as 21138-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 21138-1-AP (KIAA0528 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21138-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 21138-1-AP (same clone as 21138-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 21138-1-AP (KIAA0528 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21138-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 21138-1-AP (same clone as 21138-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using KIAA0528 antibody 21138-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 21138-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

21138-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA KIAA0528 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KIAA0528 fusion protein Ag15590
• Vollständiger Name: KIAA0528
• Berechnetes Molekulargewicht: 1000 aa, 110 kDa
• Beobachtetes Molekulargewicht: 110-125 kDa
• GenBank-Zugangsnummer: BC042498
• Gene symbol: KIAA0528
• Gene ID (NCBI): 9847
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.