KIAA1199 / CEMIP Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

KIAA1199 / CEMIP Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(49)

Rezensionen (1)

Kat-Nr. : 21129-1-AP

Synonyme

CEMIP, KIAA1199, EC:3.2.1.35, Hyaluronan binding protein involved in hyaluronan depolymerization, HYBID

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  WB analysis using 21129-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21129-1-AP (KIAA1199 / CEMIP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 21129-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21129-1-AP (KIAA1199 / CEMIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate hyperplasia using 21129-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 21129-1-AP (KIAA1199 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate hyperplasia using 21129-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 21129-1-AP (KIAA1199 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate hyperplasia using 21129-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 21129-1-AP (KIAA1199 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate hyperplasia using 21129-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 21129-1-AP (KIAA1199 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human stomach cancer using 21129-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 21129-1-AP (CEMIP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach cancer using 21129-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 21129-1-AP (CEMIP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse testis using 21129-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21129-1-AP (KIAA1199 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of mouse testis using 21129-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21129-1-AP (KIAA1199 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 21129-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KIAA1199 / CEMIP antibody (21129-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  FC experiment of HepG2 using 21129-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug KIAA1199 / CEMIP Polyclonal antibody (21129-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U-87 MG-Zellen, COLO 320-Zellen, SGC-7901-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatahyperplasie-Gewebe, humanes Magenkrebsgewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:300-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 18 publications below
• WB: See 34 publications below
• IHC: See 29 publications below
• IF: See 9 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Produktinformation

21129-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA KIAA1199 / CEMIP und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KIAA1199 / CEMIP fusion protein Ag15527
• Vollständiger Name: KIAA1199 / CEMIP
• Berechnetes Molekulargewicht: 1361 aa, 153 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: BC020256
• Gene symbol: KIAA1199
• Gene ID (NCBI): 57214
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KIAA1199, also known as CEMIP, was first discovered as an inner-ear protein whose genetic mutations were linked to nonsyndromic hearing loss. KIAA1199 is involved in hyaluronan degradation. Studies have shown that KIAA1199 is overexpressed in many types of cancers, suggesting a critical role of KIAA1199 in cancer progression.

Publikationen

Species	Application	Title
human	WB	Nat Commun; TREX tetramer disruption alters RNA processing necessary for corticogenesis in THOC6 Intellectual Disability Syndrome; Authors - Elizabeth A Werren
human	IHC	Sci Adv; Single-cell analysis of human basal cell carcinoma reveals novel regulators of tumor growth and the tumor microenvironment.; Authors - Christian F Guerrero-Juarez
human	WB	Am J Hum Genet; Altered regulation of DPF3, a member of the SWI/SNF complexes, underlies the 14q24 renal cancer susceptibility locus.; Authors - Leandro M Colli ; KD Validated
human	IHC	Theranostics; Functional Genomic mRNA Profiling of Colorectal Adenomas: Identification and in vivo Validation of CD44 and Splice Variant CD44v6 as Molecular Imaging Targets.; Authors - Elmire Hartmans
mouse,human	WB,IHC,IF,CoIP	Acta Pharmacol Sin; Adaptor protein CEMIP reduces the chemosensitivity of small cell lung cancer via activation of an SRC-YAP oncogenic module; Authors - Xiao-Ju Shen ; KD Validated
human	IF	Cell Rep; Cochlear Cell Modeling Using Disease-Specific iPSCs Unveils a Degenerative Phenotype and Suggests Treatments for Congenital Progressive Hearing Loss.; Authors - Makoto Hosoya

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

DAN (Verified Customer) (06-10-2022)

• Applications: Western Blot