KIAA1429 Monoklonaler Antikörper

KIAA1429 Monoklonal Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

3F1A4

Kat-Nr. : 68235-1-Ig

Synonyme

VIRMA, 3F1A4, MSTP054, virilizer

---

• 

  WB analysis using 68235-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68235-1-Ig (KIAA1429 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68235-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68235-1-Ig (KIAA1429 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 68235-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68235-1-Ig (KIAA1429 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of MCF-7 using 68235-1-Ig

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human KIAA1429 (68235-1-Ig, Clone:3F1A4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, 4T1-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68235-1-Ig bindet in WB, IHC, FC (Intra), ELISA KIAA1429 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: KIAA1429 fusion protein Ag22449
• Vollständiger Name: KIAA1429
• Berechnetes Molekulargewicht: 1812 aa, 202 kDa
• Beobachtetes Molekulargewicht: 200-210 kDa
• GenBank-Zugangsnummer: BC113380
• Gene symbol: KIAA1429
• Gene ID (NCBI): 25962
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VIRMA (KIAA1429) is a key component of m6A methyltransferase (writer) complex that include METTL3, METTL14, WTAP, VIRMA, HAKAI, ZC3H13, and RBM15. VIRMA mediates methylation in the 3'UTR and around the stop codon, thus affecting alternative polyadenylation. VIRMA has been reported to act as an oncogenic factor in breast cancer and liver cancer. KIAA1429 exhibits differential nuclear and cytoplasmic distribution in colorectal cancer samples, and high cytoplasmic expression of KIAA1429 is associated with poor response to chemotherapy. BRAF-mediated phosphorylation of KIAA1429 facilitates its aggregation in the cytoplasm. Furthermore, cytoplasmic KIAA1429 promotes WNT pathway activation by binding and stabilizing FZD7, thereby further enhancing cancer stemness and oxaliplatin resistance (PMID: 40611274).