KIAA1429 Monoklonaler Antikörper

KIAA1429 Monoklonal Antikörper für WB, IHC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3F1A4

Kat-Nr. : 68235-1-PBS

Synonyme

VIRMA, 3F1A4, MSTP054, virilizer

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  WB analysis using 68235-1-Ig (same clone as 68235-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68235-1-Ig (KIAA1429 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68235-1-PBS in a different storage buffer formulation.

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  WB analysis using 68235-1-Ig (same clone as 68235-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68235-1-Ig (KIAA1429 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68235-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 68235-1-Ig (same clone as 68235-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68235-1-Ig (KIAA1429 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68235-1-PBS in a different storage buffer formulation.

• 

  FC experiment of MCF-7 using 68235-1-Ig (same clone as 68235-1-PBS)

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human KIAA1429 (68235-1-Ig, Clone:3F1A4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68235-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68235-1-PBS bindet in WB, IHC, FC (Intra), Indirect ELISA KIAA1429 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: KIAA1429 fusion protein Ag22449
• Vollständiger Name: KIAA1429
• Berechnetes Molekulargewicht: 1812 aa, 202 kDa
• Beobachtetes Molekulargewicht: 200-210 kDa
• GenBank-Zugangsnummer: BC113380
• Gene symbol: KIAA1429
• Gene ID (NCBI): 25962
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VIRMA (KIAA1429) is a key component of m6A methyltransferase (writer) complex that include METTL3, METTL14, WTAP, VIRMA, HAKAI, ZC3H13, and RBM15. VIRMA mediates methylation in the 3'UTR and around the stop codon, thus affecting alternative polyadenylation. VIRMA has been reported to act as an oncogenic factor in breast cancer and liver cancer. KIAA1429 exhibits differential nuclear and cytoplasmic distribution in colorectal cancer samples, and high cytoplasmic expression of KIAA1429 is associated with poor response to chemotherapy. BRAF-mediated phosphorylation of KIAA1429 facilitates its aggregation in the cytoplasm. Furthermore, cytoplasmic KIAA1429 promotes WNT pathway activation by binding and stabilizing FZD7, thereby further enhancing cancer stemness and oxaliplatin resistance (PMID: 40611274).