Shootin 1 Polyklonaler Antikörper

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Shootin 1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 24383-1-AP

Synonyme

SHTN1, KIAA1598, Shootin-1

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  WB analysis of Neuro-2a using 24383-1-AP

  WB result of Shootin 1 antibody (24383-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Shootin 1 transfected Neuro-2a cells.

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  WB analysis using 24383-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24383-1-AP (Shootin 1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human bowen disease using 24383-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 24383-1-AP (Shootin 1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human bowen disease using 24383-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 24383-1-AP (Shootin 1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, Neuro-2a-Zellen
• Erfolgreiche Detektion in IHC: human bowen disease; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

24383-1-AP bindet in WB, IHC, ELISA Shootin 1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Shootin 1 fusion protein Ag18294
• Vollständiger Name: KIAA1598
• Berechnetes Molekulargewicht: 72 kDa
• Beobachtetes Molekulargewicht: 60-80 kDa
• GenBank-Zugangsnummer: BC022348
• Gene symbol: Shootin 1
• Gene ID (NCBI): 57698
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Shootin 1, also known as SHTN1, KIAA1598, contains 3 coiled-coil domains and a proline-rich region. Shootin 1 is involved in generating internal asymmetric signals required for neuronal during stages 2 and 3. Shootin 1 plays a role in cytoskeletal organization by regulating the subcellular localization of phosphoinositide 3-kinase (PI3K) activity at the axonal growth cone. SHTN1 produces 2 splice variants, a short variant, SHTN1S, that lacks exons 15 and 16, and a long variant, SHTN1L, that includes exons 1 through 17(PMID: 17030985, 30733148).

Publikationen

Species	Application	Title
human	WB,IHC	Cancer Genet; Data-mining-based biomarker evaluation and experimental validation of SHTN1 for bladder cancer; Authors - Yueying Wang