Shootin 1 Rekombinanter Antikörper

Shootin 1 Rekombinant Antikörper für WB, Cytometric bead array, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Cytometric bead array, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

251435E5

Kat-Nr. : 86541-2-PBS

Synonyme

SHTN1, KIAA1598, Shootin-1

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  WB analysis using 86541-2-RR (same clone as 86541-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86541-2-RR (KIAA1598 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86541-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 86541-2-RR (same clone as 86541-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86541-2-RR (KIAA1598 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86541-2-PBS in a different storage buffer formulation.

• 

  Sample test of MP02499-1

  The mean Shootin 1 concentration was determined to be 50.1 ng/mL in SH-SY5Y cell extract based on a 1.4 mg/mL extract load, 47.7 ng/mL in Neuro-2a cell extract based on a 1.6 mg/mL extract load.

• 

  Cytometric bead array standard curve of MP02499-1

  Cytometric bead array standard curve of MP02499-1, Shootin 1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86541-2-PBS. Detection antibody: 86541-1-PBS. Standard: Ag18294. Range: 0.781-100 ng/mL.

• 

  Affinity and Kinetic Characterization of 86541-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86541-2-RR against Human Shootin 1 were performed. The affinity constant is 0.965 nM.

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Geprüfte Anwendungen

Produktinformation

86541-2-PBS bindet in WB, Cytometric bead array, Indirect ELISA, Sample test Shootin 1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Shootin 1 fusion protein Ag18294
• Vollständiger Name: KIAA1598
• Berechnetes Molekulargewicht: 72 kDa
• Beobachtetes Molekulargewicht: 60-80 kDa
• GenBank-Zugangsnummer: BC022348
• Gene symbol: Shootin 1
• Gene ID (NCBI): 57698
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Shootin 1, also known as SHTN1, KIAA1598, contains 3 coiled-coil domains and a proline-rich region. Shootin 1 is involved in generating internal asymmetric signals required for neuronal during stages 2 and 3. Shootin 1 plays a role in cytoskeletal organization by regulating the subcellular localization of phosphoinositide 3-kinase (PI3K) activity at the axonal growth cone. SHTN1 produces 2 splice variants, a short variant, SHTN1S, that lacks exons 15 and 16, and a long variant, SHTN1L, that includes exons 1 through 17(PMID: 17030985, 30733148).