KIDINS220 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

KIDINS220 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Rezensionen (1)

Kat-Nr. : 21856-1-AP

Synonyme

Ankyrin repeat-rich membrane-spanning protein, ARMS, KIDINS 220, Kinase D-interacting substrate of 220 kDa

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• 

  WB analysis of HeLa using 21856-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21856-1-AP (KIDINS220 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21856-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21856-1-AP (KIDINS220 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21856-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21856-1-AP (KIDINS220 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 21856-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21856-1-AP (KIDINS220 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 21856-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21856-1-AP (KIDINS220 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 21856-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21856-1-AP (KIDINS220 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21856-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21856-1-AP (KIDINS220 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 21856-1-AP

  IP result of anti-KIDINS220 (IP:21856-1-AP, 4ug; Detection:21856-1-AP 1:3000) with HEK-293 cells lysate 1800 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, humanes Hirngewebe, Raji-Zellen
• Erfolgreiche IP: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IP: See 1 publications below

Produktinformation

21856-1-AP bindet in WB, IHC, IP, ELISA KIDINS220 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KIDINS220 fusion protein Ag16448
• Vollständiger Name: kinase D-interacting substrate, 220kDa
• Berechnetes Molekulargewicht: 1672 aa, 187 kDa
• Beobachtetes Molekulargewicht: 186-197 kDa
• GenBank-Zugangsnummer: BC130610
• Gene symbol: KIDINS220
• Gene ID (NCBI): 57498
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Kidins220 (Kinase D-interacting substrate 220 kD) is a crucial mediator of signal transduction in neural tissues. Also it named ARMS(Ankyrin Repeat-Rich Membrane Spanning) due to the presence of ankyrin repeats and transmembrane domains together with other binding motifs. It is a multidomain transmembrane protein that not only transduces signaling mediated by the receptor tyrosine kinase (RTK), but also regulates the stability of cytoskeletal network through a number of cytoskeleton-associated proteins, including Septin5, MAPs , and stathmin family members. KIDINS220 exists some isoforms with MV 194 kDa, 185 kDa, 115 kDa, and 60 kDa.

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; A global analysis of SNX27-retromer assembly and cargo specificity reveals a function in glucose and metal ion transport.; Authors - Steinberg Florian F
human	WB	J Exp Med; Kidins220/ARMS binds to the B cell antigen receptor and regulates B cell development and activation.; Authors - Gina J Fiala ; KD Validated
mouse	WB	Sci Adv; Kidins220 and Aiolos promote thymic iNKT cell development by reducing TCR signals; Authors - Laurenz A Herr
human	WB	Nat Commun; Regulation of the endosomal SNX27-retromer by OTULIN.; Authors - Aurelia Stangl
human	WB,IP	Proc Natl Acad Sci U S A; A unique PDZ domain and arrestin-like fold interaction reveals mechanistic details of endocytic recycling by SNX27-retromer.; Authors - Matthew Gallon
human		Front Immunol; Patients' IgLON5 autoantibodies interfere with IgLON5-protein interactions; Authors - Jon Landa

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kincaid (Verified Customer) (06-02-2024)

H4 cell lysate run on SDS-PAGE gel. aKIDINS220 diluted in 3% BSA in TBS-tween (0.1%).

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: H4 cells

KIDINS220 Antibody Western Blot validation (1:1000 dilution) in H4 cells (Cat no:21856-1-AP)