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KIF15 Polyklonaler Antikörper

KIF15 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30774-1-PBS

Synonyme

hKLP2, Kinesin-like protein 2, Kinesin-like protein 7, Kinesin-like protein KIF15, KLP2

Filter:
  • Western Blot (WB) analysis of various lysates using KIF15 Polyclonal antibody (30774-1-AP)

    WB analysis using 30774-1-AP (same clone as 30774-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30774-1-AP (KIF15 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30774-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using KIF15 Polyclonal antibody (30774-1-AP)

    WB analysis of HeLa using 30774-1-AP (same clone as 30774-1-PBS)

    WB result of KIF15 antibody (30774-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF15 transfected HeLa cells. This data was developed using the same antibody clone with 30774-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using KIF15 Polyclonal antibody (30774-1-AP)

    IF Staining of HEK-293 using 30774-1-AP (same clone as 30774-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using KIF15 antibody (30774-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30774-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

30774-1-PBS bindet in WB, IF/ICC, Indirect ELISA KIF15 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen KIF15 fusion protein Ag33897
Vollständiger Name kinesin family member 15
Berechnetes Molekulargewicht160 kDa
Beobachtetes Molekulargewicht145-150 kDa
GenBank-ZugangsnummerNM_020242
Gene symbol KIF15
Gene ID (NCBI) 56992
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KIF15, also named as KLP2 and KNSL7, belongs to the kinesin-like protein family and KLP2 subfamily. It is a plus-end directed kinesin-like motor enzyme which involved in mitotic spindle assembly. It is located in the cytoplasm and biased expression in testis. Several isoforms of KIF15 exist due to the alternative splicing, with molecular weight between 130-160 kDa. Sometimes higher molecular weight around 200 kDa can also be observed, which may be a modified variant of KIF15 (14618103). It is known that KIF15 participates in important events during neuronal development and involves multiple cellular processes such as proliferation, apoptosis, and differentiation. There is emerging evidence indicating that KIF15 plays an important role in several malignancies including pancreatic cancer, hepatocellular carcinoma, and breast cancer (PMID: 33277366).