KIF15 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

KIF15 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

230049F1

Kat-Nr. : 82903-1-RR

Synonyme

230049F1, hKLP2, Kinesin-like protein 2, Kinesin-like protein 7, Kinesin-like protein KIF15

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  WB analysis of HeLa using 82903-1-RR

  WB result of KIF15 antibody (82903-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF15 transfected HeLa cells.

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  WB analysis using 82903-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82903-1-RR (KIF15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 82903-1-RR

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82903-1-RR (KIF15 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 82903-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82903-1-RR against Human KIF15 were performed. The affinity constant is 75.8 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: human intrahepatic cholangiocarcinoma tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82903-1-RR bindet in WB, IHC, ELISA KIF15 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: KIF15 fusion protein Ag33897
• Vollständiger Name: kinesin family member 15
• Berechnetes Molekulargewicht: 160 kDa
• Beobachtetes Molekulargewicht: 160 kDa
• GenBank-Zugangsnummer: NM_020242
• Gene symbol: KIF15
• Gene ID (NCBI): 56992
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KIF15, also named as KLP2 and KNSL7, belongs to the kinesin-like protein family and KLP2 subfamily. It is a plus-end directed kinesin-like motor enzyme which involved in mitotic spindle assembly. Several isoforms of KIF15 exist due to the alternative splicing, with molecular weight between 130-160 kDa. Sometimes higher molecular weight around 200 kDa can also be observed, which may be a modified variant of KIF15. (14618103)