KIF20A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

KIF20A Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(13)

Rezensionen (2)

Kat-Nr. : 15911-1-AP

Synonyme

FLJ21151, GG10_2, Kinesin-like protein KIF20A, Mitotic kinesin-like protein 2, MKlp2

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• 

  WB analysis using 15911-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 15911-1-AP (KIF20A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15911-1-AP

  WB result of KIF20A antibody (15911-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF20A transfected HeLa cells.

• 

  IP experiment of HEK-293 using 15911-1-AP

  IP result of anti-KIF20A (IP:15911-1-AP, 4ug; Detection:15911-1-AP 1:500) with HEK-293 cells lysate 1200ug.

• 

  IHC staining of human breast cancer using 15911-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15911-1-AP (KIF20A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15911-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15911-1-AP (KIF20A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15911-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15911-1-AP (KIF20A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 15911-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using KIF20A antibody (15911-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Caco-2-Zellen, HeLa-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IHC: See 5 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Produktinformation

15911-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA KIF20A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KIF20A fusion protein Ag8700
• Vollständiger Name: kinesin family member 20A
• Berechnetes Molekulargewicht: 890 aa, 100 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC012999
• Gene symbol: KIF20A
• Gene ID (NCBI): 10112
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KIF20A, also known as RAB6KIFL or MKlp2, is a kinesin originally identified as a binding partner for the rab6 GTPase involved in protein transport at the Golgi apparatus. KIF20A belongs to a large family of motor proteins that accumulate in mitotic cells and is required for normal cell division. KIF20A is thought to be involved in carcinogenesis. In normal tissues KIF20A is almost undetectable while it is overexpressed in various cancers. KIF20A has been identified as a novel promising candidate for anticancer immunotherapy.

Publikationen

Species	Application	Title
human	WB,IF	Nat Commun; Coupling fission and exit of RAB6 vesicles at Golgi hotspots through kinesin-myosin interactions.; Authors - Stéphanie Miserey-Lenkei
human	WB,IHC,IF	Cancer Lett; Inhibition of KIF20A enhances the immunotherapeutic effect of hepatocellular carcinoma by enhancing c-Myc ubiquitination; Authors - Shujia Chen
human	WB,IHC,IF,CoIP	J Ginseng Res; Ginsenoside 20(S)-Rg3 reduces KIF20A expression and promotes CDC25A proteasomal degradation in epithelial ovarian cancer; Authors - Rong Zhang
human	WB	Proteomics; Genistein-induced mitotic arrest of gastric cancer cells by downregulating KIF20A, a proteomics study.; Authors - Yan Guang-Rong GR
human	IHC	J Cancer; Five-gene signature associating with Gleason score serve as novel biomarkers for identifying early recurring events and contributing to early diagnosis for Prostate Adenocarcinoma.; Authors - Lingyu Zhang
		J Biochem Mol Toxicol; Bisphenol A triggers the malignancy of acute myeloid leukemia cells via regulation of IL-4 and IL-6.; Authors - Suwei Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (04-03-2024)

Not suitable for IF at all. showed many blobs staining all over the field, and cannot label any Golgi staining.

• Applications: Immunofluorescence

FH

Juliane (Verified Customer) (10-25-2022)

Golgi and nucleus staining ok but cytoplasmic difficult

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HeLa

KIF20A Antibody Immunofluorescence validation (1:500 dilution) in HeLa (Cat no:15911-1-AP)