KIF2A Rekombinanter Antikörper

KIF2A Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

250946C7

Kat-Nr. : 86268-3-RR

Synonyme

HK2, KIF2, Kinesin 2, kinesin heavy chain member 2A, Kinesin like protein KIF2A

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  WB analysis using 86268-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86268-3-RR (KIF2A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86268-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86268-3-RR (KIF2A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86268-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86268-3-RR against Human KIF2A were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, Maushirngewebe, NCI-H1299-Zellen, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86268-3-RR bindet in WB, ELISA KIF2A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: KIF2A fusion protein Ag3748
• Vollständiger Name: kinesin heavy chain member 2A
• Berechnetes Molekulargewicht: 679 aa, 77 kDa
• Beobachtetes Molekulargewicht: 80-100 kDa
• GenBank-Zugangsnummer: BC031828
• Gene symbol: KIF2A
• Gene ID (NCBI): 3796
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Kinesin family member 2a (KIF2A), one of the kinesin family members, is identified in mammalian cells and involved in regulating microtubule dynamics during the mitosis via modulating intracellular transport, cell division, and bipolar spindle assembly (PMID: 17538014). High expression of KIF2A correlates with advanced tumor stages and worse prognosis in non-small cell lung cancer patients (PMID: 31858647). Within clinical practice, KIF2A exhibits key prognostic value for the management of gastric cancer, colorectal cancer and nasopharyngeal carcinoma (PMID: 32106376, PMID: 26070867, PMID: 26602436).