KIF5A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

KIF5A Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Rezensionen (1)

Kat-Nr. : 21186-1-AP

Synonyme

NKHC, Neuronal kinesin heavy chain, MY050, Kinesin heavy chain neuron-specific 1, Kinesin heavy chain isoform 5A

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  WB analysis using 21186-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21186-1-AP (KIF5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 21186-1-AP

  IP result of anti-KIF5A (IP:21186-1-AP, 4ug; Detection:21186-1-AP 1:2000) with mouse brain tissue lysate 1840 ug.

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  IHC staining of mouse brain using 21186-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21186-1-AP (KIF5A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21186-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21186-1-AP (KIF5A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 21186-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21186-1-AP (KIF5A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 21186-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21186-1-AP (KIF5A antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of PC-12 using 21186-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using KIF5A antibody (21186-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: PC-12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

21186-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA KIF5A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KIF5A fusion protein Ag15518
• Vollständiger Name: kinesin family member 5A
• Berechnetes Molekulargewicht: 1032 aa, 117 kDa
• Beobachtetes Molekulargewicht: 118-120 kDa
• GenBank-Zugangsnummer: BC146670
• Gene symbol: KIF5A
• Gene ID (NCBI): 3798
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Kinesin superfamily proteins (KIFs) are microtubule-based molecular motors essential for the intracellular transport of various cargos, including organelles, proteins, and RNAs. KIF5A is expressed exclusively in neurons and transports neuronal cargoes into axons and dendrites. KIF5A mutations have been associated with Charcot-Marie-Tooth Type 2, an axonal peripheral neuropathy characterized by progressive loss of peripheral sensation and muscle wasting.

Publikationen

Species	Application	Title
mouse	WB	Exp Neurol; Axonal injury mediated by neuronal p75NTR/TRAF6/JNK pathway contributes to cognitive impairment after repetitive mTBI; Authors - Gang Liu
human	WB	Hum Mol Genet; Mitochondrial transport mediates survival of retinal ganglion cells in affected LHON patients.; Authors - Tien-Chun Yang
rat	WB	Biol Sex Differ; Axonal transport in a peripheral diabetic neuropathy model: sex-dimorphic features.; Authors - Marzia Pesaresi
mouse	IF	Cell Rep Methods; Human MAPT knockin mouse models of frontotemporal dementia for the neurodegenerative research community; Authors - Takahiro Morito
mouse	WB	Alzheimers Res Ther; Normal levels of KIF5 but reduced KLC1 levels in both Alzheimer disease and Alzheimer disease in Down syndrome: evidence suggesting defects in anterograde transport.; Authors - Xu-Qiao Chen
mouse	WB	JCI Insight; Transcriptional control of a collagen deposition and adhesion process that promotes lung adenocarcinoma growth and metastasis.; Authors - Xiaochao Tan ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xuqiao (Verified Customer) (08-25-2020)

It specifically probes both endogenous and transfected KIF5A from either human or mouse.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293T cell

KIF5A Antibody Western Blot validation (1:2000 dilution) in HEK293T cell (Cat no:21186-1-AP)