KIF5A Polyklonaler Antikörper

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• KD/KO Validated

KIF5A Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21186-1-PBS

Synonyme

NKHC, Neuronal kinesin heavy chain, MY050, Kinesin heavy chain neuron-specific 1, Kinesin heavy chain isoform 5A

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  WB analysis using 21186-1-AP (same clone as 21186-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21186-1-AP (KIF5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21186-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 21186-1-AP (same clone as 21186-1-PBS)

  IP result of anti-KIF5A (IP:21186-1-AP, 4ug; Detection:21186-1-AP 1:2000) with mouse brain tissue lysate 1840 ug. This data was developed using the same antibody clone with 21186-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 21186-1-AP (same clone as 21186-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21186-1-AP (KIF5A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21186-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 21186-1-AP (same clone as 21186-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21186-1-AP (KIF5A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21186-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 21186-1-AP (same clone as 21186-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 21186-1-AP (KIF5A antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 21186-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 21186-1-AP (same clone as 21186-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 21186-1-AP (KIF5A antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 21186-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-12 using 21186-1-AP (same clone as 21186-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using KIF5A antibody (21186-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 21186-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

21186-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA KIF5A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KIF5A fusion protein Ag15518
• Vollständiger Name: kinesin family member 5A
• Berechnetes Molekulargewicht: 1032 aa, 117 kDa
• Beobachtetes Molekulargewicht: 118-120 kDa
• GenBank-Zugangsnummer: BC146670
• Gene symbol: KIF5A
• Gene ID (NCBI): 3798
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Kinesin superfamily proteins (KIFs) are microtubule-based molecular motors essential for the intracellular transport of various cargos, including organelles, proteins, and RNAs. KIF5A is expressed exclusively in neurons and transports neuronal cargoes into axons and dendrites. KIF5A mutations have been associated with Charcot-Marie-Tooth Type 2, an axonal peripheral neuropathy characterized by progressive loss of peripheral sensation and muscle wasting.