KIF5A Rekombinanter Antikörper

KIF5A Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241124H1

Kat-Nr. : 84013-4-PBS

Synonyme

241124H1, D12S1889, Kinesin heavy chain isoform 5A, Kinesin heavy chain neuron-specific 1, MY050

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  WB analysis of rat brain using 84013-4-RR (same clone as 84013-4-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 84013-4-RR (KIF5A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84013-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 84013-4-RR (same clone as 84013-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84013-4-RR (KIF5A antibody) at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84013-4-PBS in a different storage buffer formulation.

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  IF Staining of PC-12 using 84013-4-RR (same clone as 84013-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using KIF5A antibody (84013-4-RR, Clone: 241124H1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84013-4-PBS in a different storage buffer formulation.

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  FC experiment of K-562 using 84013-4-RR (same clone as 84013-4-PBS)

  1x10^6 K-562 cells were intracellularly stained with 0.25 ug KIF5A Recombinant antibody (84013-4-RR, Clone:241124H1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84013-4-PBS in a different storage buffer formulation.

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  FC experiment of SH-SY5Y using 84013-4-RR (same clone as 84013-4-PBS)

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug KIF5A Recombinant antibody (84013-4-RR, Clone:241124H1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84013-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84013-4-PBS

  Biolayer interferometry (BLl) kinetic assay of 84013-4-PBS against Human KIF5A was performed. The affinity constant is 0.611 nM.

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  Affinity and Kinetic Characterization of 84013-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84013-4-RR against Human KIF5A were performed. The affinity constant is 78.9 pM.

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Geprüfte Anwendungen

Produktinformation

84013-4-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA KIF5A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: KIF5A fusion protein Ag15518
• Vollständiger Name: kinesin family member 5A
• Berechnetes Molekulargewicht: 1032 aa, 117 kDa
• Beobachtetes Molekulargewicht: 118-120 kDa
• GenBank-Zugangsnummer: BC146670
• Gene symbol: KIF5A
• Gene ID (NCBI): 3798
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Kinesin superfamily proteins (KIFs) are microtubule-based molecular motors essential for the intracellular transport of various cargos, including organelles, proteins, and RNAs. KIF5A is expressed exclusively in neurons and transports neuronal cargoes into axons and dendrites. KIF5A mutations have been associated with Charcot-Marie-Tooth Type 2, an axonal peripheral neuropathy characterized by progressive loss of peripheral sensation and muscle wasting.