KIF5B Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

KIF5B Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2B18

Kat-Nr. : 82485-1-PBS

Synonyme

KNS1, KNS, KINH, Kinesin-1 heavy chain, KIF 5B

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  WB analysis using 82485-1-RR (same clone as 82485-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82485-1-RR (KIF5B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82485-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 82485-1-RR (same clone as 82485-1-PBS)

  WB result of KIF5B antibody (82485-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF5B transfected HeLa cells. This data was developed using the same antibody clone with 82485-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovarian cancer using 82485-1-RR (same clone as 82485-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82485-1-RR (KIF5B antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82485-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 82485-1-RR (same clone as 82485-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using KIF5B antibody (82485-1-RR, Clone: 2B18 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82485-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

82485-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA KIF5B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: KIF5B fusion protein Ag16250
• Vollständiger Name: kinesin family member 5B
• Berechnetes Molekulargewicht: 963 aa, 110 kDa
• Beobachtetes Molekulargewicht: 110-120 kDa
• GenBank-Zugangsnummer: BC126279
• Gene symbol: KIF5B
• Gene ID (NCBI): 3799
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KIF5B, also known as KINH, KNS1, belongs to the Kinesin-1 family. KIF5B contains three structural domains: a globular N-terminal motor domain, a central alpha-helical rod domain, and a globular C-terminal domain. During the G2 phase of the cell cycle in a BICD2-dependent manner, KIF5B antagonizes the dynein function and drives the separation of nuclei and centrosomes (PMID: 20386726). KIF5B drives the polarization of cytolytic granules and microtubule-organizing centers (MTOCs) toward the immune synapse between effector NK lymphocytes and target cells (KIF5B:24088571).