KIF7 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

KIF7 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 24693-1-AP

Synonyme

KIF7, kinesin family member 7, Kinesin like protein KIF7, UNQ340

---

• 

  WB analysis of HEK-293 using 24693-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24693-1-AP (KIF7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24693-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24693-1-AP (KIF7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 24693-1-AP

  IP result of anti-KIF7 (IP:24693-1-AP, 5ug; Detection:24693-1-AP 1:500) with HEK-293 cells lysate 1760ug.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen
• Erfolgreiche IP: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

24693-1-AP bindet in WB, IHC, IF, IP, ELISA KIF7 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KIF7 fusion protein Ag19119
• Vollständiger Name: kinesin family member 7
• Berechnetes Molekulargewicht: 1343 aa, 151 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC112271
• Gene symbol: KIF7
• Gene ID (NCBI): 374654
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KIF7(Kinesin family member 7) is a microtubule interacting protein that functions to regulate Hh signaling by maintaining the architecture of the primary cilium, a complex microtubule-based organelle with many signal transduction functions (PMID: 26439735). KIF7 has been shown to have both negative and positive roles in Shh signal transduction. Without a ligand, KIF7 localizes to the cilium base where it forms a complex with Gli proteins and other pathway components and promotes the processing of GliRs (PMID: 21552264).

Publikationen

Species	Application	Title
human	IF	Sci Adv; Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.; Authors - Chuan Chen
human	WB	Cells; Determination of WWOX Function in Modulating Cellular Pathways Activated by AP-2α and AP-2γ Transcription Factors in Bladder Cancer.; Authors - Damian Kołat
human	WB	Thorac Cancer; Human papillomavirus 16 (HPV 16) E6 but not E7 inhibits the antitumor activity of LKB1 in lung cancer cells by downregulating the expression of KIF7.; Authors - Yue Hu ; KD Validated
human	WB,IHC	Cell Death Dis; Circular RNA circUBE2J2 acts as the sponge of microRNA-370-5P to suppress hepatocellular carcinoma progression.; Authors - Lu Zhang
human	WB	Proteome Sci; Proteomics identifies differentially expressed proteins in glioblastoma U87 cells treated with hederagenin; Authors - Yesen Zhang
mouse,human	WB,IF	Leukemia; P4HA2 hydroxylates SUFU to regulate the paracrine Hedgehog signaling and promote B-cell lymphoma progression; Authors - Quanfu Li