KIFAP3 Polyklonaler Antikörper

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• KD/KO Validated

KIFAP3 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 12700-1-AP

Synonyme

KAP 3, KAP3, KIF3AP, KIFAP3, kinesin associated protein 3, SMAP, Smg GDS, Smg GDS associated protein

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Galerie der Validierungsdaten

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  WB analysis of human brain using 12700-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12700-1-AP (KIFAP3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12700-1-AP

  WB result of KIFAP3 antibody (12700-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIFAP3 transfected MCF-7 cells.

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  WB analysis of mouse testis using 12700-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12700-1-AP (KIFAP3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12700-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12700-1-AP (KIFAP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12700-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 12700-1-AP (KIFAP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 12700-1-AP

  IP Result of anti-KIFAP3 (IP:12700-1-AP, 4ug; Detection:12700-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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  IF Staining of HepG2 using 12700-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 12700-1-AP (KIFAP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, Maushirngewebe, Maushodengewebe, MCF-7-Zellen
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

12700-1-AP bindet in WB, IP, IF, ELISA KIFAP3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KIFAP3 fusion protein Ag3394
• Vollständiger Name: kinesin-associated protein 3
• Berechnetes Molekulargewicht: 792 aa, 91 kDa
• Beobachtetes Molekulargewicht: 91-100 kDa
• GenBank-Zugangsnummer: BC028679
• Gene symbol: KIFAP3
• Gene ID (NCBI): 22920
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KIFAP3, also known as KIF3AP or KAP3, is a novel KIF3A/3B-associated protein. It binds to the tail domain of KIF3A/3B and may play a role in regulating the binding of KIF3A/3B to cargoes. Recently it has been reported that mutation within the KIFAP3 gene is associated with decreased KIFAP3 expression and increased survival in sporadic ALS, which makes it a potential target for ALS therapy. KIFAP3 has also been identified as the target of mir-130a.

Publikationen

Species	Application	Title
human	WB	Nucleic Acids Res; A functional assay for microRNA target identification and validation.; Authors - G?ken Joop J
human	WB	Commun Biol; The germline factor DDX4 contributes to the chemoresistance of small cell lung cancer cells; Authors - Christopher Noyes