KIFC1 Rekombinanter Antikörper

KIFC1 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250588F10

Kat-Nr. : 85981-1-PBS

Synonyme

HSET, Kinesin-like protein 2, Kinesin-like protein KIFC1, Kinesin-related protein HSET, KNSL2

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  WB analysis of HeLa using 85981-1-RR (same clone as 85981-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 85981-1-RR (KIFC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85981-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 85981-1-RR (same clone as 85981-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 85981-1-RR (KIFC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85981-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 85981-1-RR (same clone as 85981-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 85981-1-RR (KIFC1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85981-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 85981-1-RR (same clone as 85981-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 85981-1-RR (KIFC1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85981-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 85981-1-RR (same clone as 85981-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 85981-1-RR (KIFC1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85981-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 85981-1-RR (same clone as 85981-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 85981-1-RR (KIFC1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85981-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 85981-1-RR (same clone as 85981-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85981-1-RR (KIFC1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85981-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 85981-1-RR (same clone as 85981-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85981-1-RR (KIFC1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85981-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 85981-1-RR (same clone as 85981-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KIFC1 antibody (85981-1-RR, Clone: 250588F10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85981-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85981-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85981-1-RR against Human KIFC1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

85981-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA KIFC1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: KIFC1 fusion protein Ag14492
• Vollständiger Name: kinesin family member C1
• Berechnetes Molekulargewicht: 673 aa, 74 kDa
• Beobachtetes Molekulargewicht: 74 kDa
• GenBank-Zugangsnummer: BC000712
• Gene symbol: KIFC1
• Gene ID (NCBI): 3833
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KIFC1 is a molecular motor protein belonging to the kinesin-14 family that has motor domains at the C-terminus. KIFC1 is widely distributed, with highest expression in testis and participating in acrosome biogenesis and vesicle transport. It is also abundant in ovary, spleen, and liver. KIFC1 proteins are generally localized in the perinuclear region, and have been found to be involved chromosome alignment, congression and intracellular DNA transport (PMID: 12826589, PMID: 15236353, PMID: 23543461).