KIR2DL3/CD158b2 Rekombinanter Antikörper

KIR2DL3/CD158b2 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

242282E11

Kat-Nr. : 98373-3-RR

Synonyme

KIR2DL3, Killer cell immunoglobulin-like receptor 2DL3, GL183, CD158B2, CD158b

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  FC experiment of human PBMCs using 98373-3-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human KIR2DL3/CD158b2 Rabbit RecAb (98373-3-RR, Clone: 242282E11) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human NCAM1/CD56. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 98373-3-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human KIR2DL3/CD158b2 Rabbit RecAb (98373-3-RR, Clone: 242282E11) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98373-3-RR bindet in FC KIR2DL3/CD158b2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 3
• Berechnetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: NM_015868.2
• Gene symbol: KIR2DL3
• Gene ID (NCBI): 3804
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

Killer cell immunoglobulin-like receptors (KIRs) are a diverse family of inhibitory and activating receptors expressed on NK cells and a subset of T cells (PMID: 11861603). These polymorphic receptors interact with specific motifs on HLA class I molecules, modulate NK cytolytic activity and are encoded by genes located on chromosome 19q13.4 (PMID: 17069649). KIR2DL3, also known as CD158B2 or NKAT2, is an inhibitory receptor that is specific for HLA-C alleles (HLA-Cw1, HLA-Cw3 and HLA-Cw7) (PMID: 10196125). It is a 341-amino acid transmembrane glycoprotein consisting of an extracellular region containing two C2-type Ig-like domains, a 19-amino acid hydrophobic transmembrane region, and a long cytoplasmic tail with two immunoreceptor tyrosine-based inhibitory motifs (ITIMs). KIR2DL3 inhibits the cytolytic activity of NK cells thus preventing cell lysis (PMID: 7724594).