KIR2DL3/CD158b2 Rekombinanter Antikörper

KIR2DL3/CD158b2 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

242282E11

Kat-Nr. : CL647-98373-3

Synonyme

KIR2DL3, 242282E11, CD158 antigen-like family member B2, CD158b, CD158B2

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  FC experiment of human PBMCs using CL647-98373-3

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human KIR2DL3/CD158b2 Rabbit RecAb (CL594-98373-3, Clone: 242282E11), and 5 ul CoraLite® Plus 647 Anti-Human KIR2DL3/CD158b2 Rabbit RecAb (CL647-98373-3, Clone: 242282E11) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL647-98373-3

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human KIR2DL3/CD158b2 Rabbit RecAb (CL647-98373-3, Clone: 242282E11) (red) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-98373-3 bindet in FC KIR2DL3/CD158b2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: KIR2DL3/CD158b2 fusion protein Eg2699
• Vollständiger Name: killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 3
• Berechnetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: NM_015868.2
• Gene symbol: KIR2DL3
• Gene ID (NCBI): 3804
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Killer cell immunoglobulin-like receptors (KIRs) are a diverse family of inhibitory and activating receptors expressed on NK cells and a subset of T cells (PMID: 11861603). These polymorphic receptors interact with specific motifs on HLA class I molecules, modulate NK cytolytic activity and are encoded by genes located on chromosome 19q13.4 (PMID: 17069649). KIR2DL3, also known as CD158B2 or NKAT2, is an inhibitory receptor that is specific for HLA-C alleles (HLA-Cw1, HLA-Cw3 and HLA-Cw7) (PMID: 10196125). It is a 341-amino acid transmembrane glycoprotein consisting of an extracellular region containing two C2-type Ig-like domains, a 19-amino acid hydrophobic transmembrane region, and a long cytoplasmic tail with two immunoreceptor tyrosine-based inhibitory motifs (ITIMs). KIR2DL3 inhibits the cytolytic activity of NK cells thus preventing cell lysis (PMID: 7724594).