KISS1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

KISS1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (1)

Kat-Nr. : 18375-1-AP

Synonyme

KiSS 1, KiSS 1 metastasis suppressor, KISS1, Kisspeptin 1, Metastasis suppressor KiSS 1, metastin

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Galerie der Validierungsdaten

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  WB analysis of NIH/3T3 using 18375-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18375-1-AP (KISS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 18375-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18375-1-AP (KISS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 18375-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 18375-1-AP (KISS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 18375-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18375-1-AP (KISS1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 18375-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18375-1-AP (KISS1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human lung cancer using 18375-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 18375-1-AP (KISS1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of NIH/3T3 using 18375-1-AP

  Immunofluorescent analysis of NIH/3T3 cells using 18375-1-AP (KISS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, humanes Plazenta-Gewebe, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:100-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 3 publications below

Produktinformation

18375-1-AP bindet in WB, IHC, IF, ELISA KISS1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KISS1 fusion protein Ag13240
• Vollständiger Name: KiSS-1 metastasis-suppressor
• Berechnetes Molekulargewicht: 15 kDa
• Beobachtetes Molekulargewicht: 16-20 kDa
• GenBank-Zugangsnummer: BC022819
• Gene symbol: KISS1
• Gene ID (NCBI): 3814
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KISS1, also known as kisspeptin, is a metastasis suppressor protein that suppresses metastases of human melanomas and breast carcinomas without affecting tumorigenicity. It may regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. A C-terminally amidated peptide of KISS1, metastin (kisspeptin-54 or kp-54), functions as the endogenous ligand of the G-protein coupled receptor GPR54 (PMID: 11457843; 11385580). KISS1-GPR54 signaling is involved in the regulation of reproduction, playing an important role in initiating secretion of gonadotropin-releasing hormone (GnRH) at puberty (PMID: 18753370).

Publikationen

Species	Application	Title
human	WB,IHC	Int J Cancer; Long noncoding RNA TC0101441 induces epithelial-mesenchymal transition in epithelial ovarian cancer metastasis by downregulating KiSS1.; Authors - Jun-Jun Qiu ; KD Validated
human	IHC	Histochem Cell Biol; Functional analysis of miR-767-5p during the progression of hepatocellular carcinoma and the clinical relevance of its dysregulation.; Authors - Lei Zhang
human	IHC	Oncol Lett; Analysis of the association of the expression of KiSS-1 in colorectal cancer tissues with the pathology and prognosis.; Authors - Xinkai Huo
human	WB	Mol Oncol; Ryanodine receptor 2 promotes colorectal cancer metastasis by the ROS/BACH1 axis; Authors - Tianwei Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kristina (Verified Customer) (09-18-2020)

Works great for us- seeing an increase in modified MDA231 cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: MDA-MB-231 with modifications