KISS1 Polyklonaler Antikörper

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• KD/KO Validated

KISS1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18375-1-PBS

Synonyme

KiSS 1, KiSS 1 metastasis suppressor, Kisspeptin 1, Kisspeptin-1, Metastasis suppressor KiSS 1

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  WB analysis using 18375-1-AP (same clone as 18375-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18375-1-AP (KISS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18375-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 18375-1-AP (same clone as 18375-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18375-1-AP (KISS1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18375-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 18375-1-AP (same clone as 18375-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18375-1-AP (KISS1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18375-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 18375-1-AP (same clone as 18375-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18375-1-AP (KISS1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18375-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 18375-1-AP (same clone as 18375-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18375-1-AP (KISS1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18375-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 18375-1-AP (same clone as 18375-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 18375-1-AP (KISS1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18375-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 18375-1-PBS

  Immunofluorescent analysis of NIH/3T3 cells using 18375-1-AP (KISS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

Produktinformation

18375-1-PBS bindet in WB, IHC, ELISA KISS1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KISS1 fusion protein Ag13240
• Vollständiger Name: KiSS-1 metastasis-suppressor
• Berechnetes Molekulargewicht: 15 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC022819
• Gene symbol: KISS1
• Gene ID (NCBI): 3814
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KISS1, also known as kisspeptin, is a metastasis suppressor protein that suppresses metastases of human melanomas and breast carcinomas without affecting tumorigenicity. It may regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. A C-terminally amidated peptide of KISS1, metastin (kisspeptin-54 or kp-54), functions as the endogenous ligand of the G-protein coupled receptor GPR54 (PMID: 11457843; 11385580). KISS1-GPR54 signaling is involved in the regulation of reproduction, playing an important role in initiating secretion of gonadotropin-releasing hormone (GnRH) at puberty (PMID: 18753370).