KISS1R-Specific Polyklonaler Antikörper

KISS1R-Specific Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15505-1-PBS

Synonyme

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  WB analysis of mouse cerebellum using 15505-1-AP (same clone as 15505-1-PBS)

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 15505-1-AP (same clone as 15505-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 15505-1-AP (same clone as 15505-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 15505-1-AP (same clone as 15505-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 15505-1-AP (same clone as 15505-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 15505-1-AP (same clone as 15505-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 15505-1-AP (same clone as 15505-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 15505-1-AP (same clone as 15505-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using KISS1R antibody 15505-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 15505-1-AP (same clone as 15505-1-PBS)

  Immunofluorescent analysis of Hela cells, using KISS1R antibody 15505-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 15505-1-AP (same clone as 15505-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using KISS1R antibody 15505-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15505-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA KISS1R-Specific und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: KISS1 receptor
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 75 kDa, 43-45 kDa
• GenBank-Zugangsnummer: NM_032551
• Gene symbol: KISS1R
• Gene ID (NCBI): 84634
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KISS1R is the receptor for metastin (kisspeptin-54, 13, 14, 10), C- terminally amidated peptides of KISS-1. KISS-1 is a metastasis suppressor protein that suppresses metastases in malignant melanomas and in some other carcinomas without affecting tumorigenicity. The metastasis suppressor properties may be mediated in part by cell cycle arrest and induction of apoptosis in malignant cells. The receptor is also probably involved in the regulation and fine-tuning of trophoblast invasion generated by the trophoblast itself. Analysis of the transduction pathways activated by the receptor identifies coupling to phospholipase C and intracellular calcium release through pertussis toxin-insensitive G(q) proteins.