KLC1 Polyklonaler Antikörper

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• KD/KO Validated

KLC1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19028-1-PBS

Synonyme

KLC, KLC 1, KNS2, KNS2A

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  WB analysis using 19028-1-AP (same clone as 19028-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19028-1-AP (KLC1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19028-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 19028-1-AP (same clone as 19028-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19028-1-AP (KLC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19028-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 19028-1-AP (same clone as 19028-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 19028-1-AP (KLC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19028-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 19028-1-AP (same clone as 19028-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19028-1-AP (KLC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19028-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 19028-1-AP (same clone as 19028-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19028-1-AP (KLC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19028-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 19028-1-AP (same clone as 19028-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 19028-1-AP (KLC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19028-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 19028-1-AP (same clone as 19028-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19028-1-AP (KLC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19028-1-PBS in a different storage buffer formulation.

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  IHC staining of human bowen disease using 19028-1-AP (same clone as 19028-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 19028-1-AP (KLC1 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19028-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 19028-1-AP (same clone as 19028-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using KLC1 antibody (19028-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 19028-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 19028-1-AP (same clone as 19028-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using KLC1 antibody (19028-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 19028-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 19028-1-AP (same clone as 19028-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using KLC1 antibody 19028-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 19028-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 19028-1-AP (same clone as 19028-1-PBS)

  Immunofluorescent analysis of Hela cells, using KLC1 antibody 19028-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 19028-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

19028-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA KLC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KLC1 fusion protein Ag13570
• Vollständiger Name: kinesin light chain 1
• Berechnetes Molekulargewicht: 65 kDa
• Beobachtetes Molekulargewicht: 61-70 kDa
• GenBank-Zugangsnummer: BC008881
• Gene symbol: KLC1
• Gene ID (NCBI): 3831
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. Kinesin is tetrameric proteins containing two heavy (alpha) chains and two light (beta) chains. The alpha chains provide the tubulin binding site and the ATPase domains, whereas the beta chains are responsible for the specific attachment of the organelle to be moved by the kinesin tetramer (PubMed:21385839).