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  • Featured Product
  • KD/KO Validated

KLF15 Monoklonaler Antikörper

KLF15 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgM

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2B8G9

Kat-Nr. : 66185-1-PBS

Synonyme

DKFZp779M1320, KKLF, KLF15, Krueppel like factor 15, Kruppel like factor 15

Filter:
  • Western Blot (WB) analysis of human skeletal muscle tissue using KLF15 Monoclonal antibody (66185-1-Ig)

    WB analysis of human skeletal muscle using 66185-1-Ig (same clone as 66185-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66185-1-Ig (KLF15 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66185-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using KLF15 Monoclonal antibody (66185-1-Ig)

    WB analysis using 66185-1-Ig (same clone as 66185-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66185-1-Ig (KLF15 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66185-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KLF15 Monoclonal antibody (66185-1-Ig)

    IF Staining of HepG2 using 66185-1-Ig (same clone as 66185-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KLF15 antibody (66185-1-Ig, Clone: 2B8G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66185-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

66185-1-PBS bindet in WB, IF/ICC, ELISA KLF15 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgM
Klonalität Monoklonal
Typ Antikörper
Immunogen KLF15 fusion protein Ag4864
Vollständiger Name Kruppel-like factor 15
Berechnetes Molekulargewicht 416 aa, 44 kDa
Beobachtetes Molekulargewicht 44 kDa
GenBank-ZugangsnummerBC036733
Gene symbol KLF15
Gene ID (NCBI) 28999
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Thiophiler Affinitätschromatograph
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KLF15, a kidney-enriched Krüppel-like zinc finger-containing transcription factors, is a transcriptional activator that binds the CLCNKA promoter. The nuclear protein KLF15, is expressed primarily in liver, heart, skeletal muscle and kidney tissues but is not detected in lymphoid tissues or bone marrow. It is an important regulator of GLUT4 in both adipose and muscle tissues.(PMID:10982849,PMID:16615998).