KLF5 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

KLF5 Monoklonal Antikörper für Indirect ELISA, WB

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

2C11C5

Kat-Nr. : 66850-1-PBS

Synonyme

Colon krueppel-like factor, CKLF, BTE-binding protein 2, BTEB2, Basic transcription element-binding protein 2

---

• 

  WB analysis using 66850-1-Ig (same clone as 66850-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66850-1-Ig (KLF5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66850-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66850-1-Ig (same clone as 66850-1-PBS)

  WB result of KLF5 antibody (66850-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KLF5 transfected HeLa cells. This data was developed using the same antibody clone with 66850-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 66850-1-Ig (same clone as 66850-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human KLF5 (66850-1-Ig, Clone:2C11C5) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66850-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

66850-1-PBS bindet in WB, IHC, FC (Intra), Indirect ELISA KLF5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: KLF5 fusion protein Ag15403
• Vollständiger Name: Kruppel-like factor 5 (intestinal)
• Berechnetes Molekulargewicht: 457 aa, 51 kDa
• Beobachtetes Molekulargewicht: 51-57 kDa
• GenBank-Zugangsnummer: BC042131
• Gene symbol: KLF5
• Gene ID (NCBI): 688
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KLF5, also known as BTEB2 and IKLF, is a member of the Krueppel-like transcription factor family. It is a transcription factor for SMemb/non-muscle myosin heavy chain-B (NMHC-B). It is a molecular marker of phenotypically modulated smooth-muscle cells. KLF5 seems to be a key element linking external stress and cardiovascular remodeling.

Publikationen

Species	Application	Title
human	IHC	Adv Sci (Weinh); Positive Feedback Regulation between KLF5 and XPO1 Promotes Cell Cycle Progression of Basal like Breast Cancer; Authors - Yu Tang