KLF5 Rekombinanter Antikörper

KLF5 Rekombinant Antikörper für IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

230509G7

Kat-Nr. : 83205-4-RR

Synonyme

Colon krueppel-like factor, CKLF, BTE-binding protein 2, BTEB2, Basic transcription element-binding protein 2

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  IF Staining of HeLa using 83205-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KLF5 antibody (83205-4-RR, Clone: 230509G7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of A549 using 83205-4-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug Klf5 Recombinant Antibody (83205-4-RR, Clone:230509G7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of HT-29 using 83205-4-RR

  1x10^6 HT-29 cells were intracellularly stained with 0.25 ug Klf5 Recombinant Antibody (83205-4-RR, Clone:230509G7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83205-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83205-4-RR against Human KLF5 were performed. The affinity constant is 2.36 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): A549-Zellen, HT-29-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83205-4-RR bindet in IF/ICC, FC (Intra), ELISA KLF5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: KLF5 fusion protein Ag15267
• Vollständiger Name: Kruppel-like factor 5 (intestinal)
• Berechnetes Molekulargewicht: 457 aa, 51 kDa
• Beobachtetes Molekulargewicht: 51-57 kDa
• GenBank-Zugangsnummer: BC042131
• Gene symbol: KLF5
• Gene ID (NCBI): 688
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KLF5, also known as BTEB2 and IKLF, is a member of the Krueppel-like transcription factor family. It is a transcription factor for SMemb/non-muscle myosin heavy chain-B (NMHC-B). It is a molecular marker of phenotypically modulated smooth-muscle cells. KLF5 seems to be a key element linking external stress and cardiovascular remodeling.