KLF5 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

KLF5 Polyklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-21017

Synonyme

GC-box-binding protein 2, Colon krueppel-like factor, CKLF, BTE-binding protein 2, BTEB2

---

• 

  IF Staining of HeLa using CL488-21017

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 KLF5 antibody (CL488-21017) at dilution of 1:200, CL594-Phalloidin (red).

• 

  FC experiment of A549 using CL488-21017

  1x10^6 A549 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Klf5 Polyclonal Antibody (CL488-21017)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): A549-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-21017 bindet in IF/ICC, FC (Intra) KLF5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KLF5 fusion protein Ag15267
• Vollständiger Name: Kruppel-like factor 5 (intestinal)
• Berechnetes Molekulargewicht: 457 aa, 51 kDa
• Beobachtetes Molekulargewicht: 51-55 kDa
• GenBank-Zugangsnummer: BC042131
• Gene symbol: KLF5
• Gene ID (NCBI): 688
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KLF5, also known as BTEB2 and IKLF, is a member of the Krueppel-like transcription factor family. It is a transcription factor for SMemb/non-muscle myosin heavy chain-B (NMHC-B). It is a molecular marker of phenotypically modulated smooth-muscle cells. KLF5 seems to be a key element linking external stress and cardiovascular remodeling. This antibody detects the 50-60 kDa isoform 1 of KLF5. The antibody can recognize isoform 2 (33 kDa) and isoform 3 (18 kDa) too. 85 kDa, 95 kDa and 120 kDa of SUMOylated KLF5 were detected (PMID: 18500350).