KLHDC3 Rekombinanter Antikörper

KLHDC3 Rekombinant Antikörper für WB, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241475G5

Kat-Nr. : 84217-4-PBS

Synonyme

241475G5, hPEAS, Kelch domain-containing protein 3, PEAS

---

• 

  WB analysis using 84217-4-RR (same clone as 84217-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84217-4-RR (KLHDC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84217-4-PBS in a different storage buffer formulation.

• 

  FC experiment of PC-3 using 84217-4-RR (same clone as 84217-4-PBS)

  1x10^6 PC-3 cells were intracellularly stained with 0.25 ug KLHDC3 Recombinant antibody (84217-4-RR, Clone:241475G5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84217-4-PBS in a different storage buffer formulation.

• 

  FC experiment of U-251 using 84217-4-RR (same clone as 84217-4-PBS)

  1x10^6 U-251 cells were intracellularly stained with 0.25 ug KLHDC3 Recombinant antibody (84217-4-RR, Clone:241475G5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84217-4-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01151-2

  Cytometric bead array standard curve of MP01151-2, KLHDC3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84217-4-PBS. Detection antibody: 84217-2-PBS. Standard: Ag4879. Range: 0.781-100 ng/mL.

• 

  Cytometric bead array standard curve of MP01151-3

  Cytometric bead array standard curve of MP01151-3, KLHDC3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84217-4-PBS. Detection antibody: 84217-1-PBS. Standard: Ag4879. Range: 0.781-100 ng/mL.

• 

  Affinity and Kinetic Characterization of 84217-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84217-4-RR against Human KLHDC3 were performed. The affinity constant is below 1 pM.

---

Geprüfte Anwendungen

Produktinformation

84217-4-PBS bindet in WB, FC (Intra), Cytometric bead array, Indirect ELISA KLHDC3 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: KLHDC3 fusion protein Ag4879
• Vollständiger Name: kelch domain containing 3
• Berechnetes Molekulargewicht: 382 aa, 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC041793
• Gene symbol: KLHDC3
• Gene ID (NCBI): 116138
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Kelch domain-containing protein 3 (KLHDC3), also designated PEAS, contains 5 Kelch repeats and may be involved in meiotic recombination process. The gene encoding KLHDC3 maps to chromosome 6, which contains around 1,200 genes within 170 million base pairs of sequence. Deletion of a portion of the q arm of chromosome 6 is associated with early onset intestinal cancer suggesting the presence of a cancer susceptibility locus. Porphyria cutanea tarda, Parkinson's disease, Stickler syndrome, 21-hydroxylase deficiency and maple syrup urine disease are also associated with genes on chromosome 6. KLHDC3 protein is mainly expressed in cytoplasm, also found in meiotic chromatin.