KLHL14 Polyklonaler Antikörper

KLHL14 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29253-1-AP

Synonyme

Protein interactor of Torsin-1A, Printor, Protein interactor of torsinA, KIAA1384, Kelch-like protein 14

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  WB analysis using 29253-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29253-1-AP (KLHL14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 29253-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 29253-1-AP (KLHL14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 29253-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using KLHL14 antibody (29253-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

29253-1-AP bindet in WB, IF/ICC, ELISA KLHL14 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KLHL14 fusion protein Ag29188
• Vollständiger Name: kelch-like 14 (Drosophila)
• Berechnetes Molekulargewicht: 44 kDa, 71 kDa
• Beobachtetes Molekulargewicht: 43 kDa, 70 kDa
• GenBank-Zugangsnummer: BC021267
• Gene symbol: KLHL14
• Gene ID (NCBI): 57565
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Kelch-like protein 14 (KLHL14) is a member of the KLHL family. The kelch motif was initially discovered in Kelch. In this protein there are six copies of the motif. It has been shown for one member that it is related to Galactose Oxidase for which a structure has been solved. Two isoforms of this protein exist - 70kDa and 43kDa.