KLHL14-Specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

KLHL14-Specific Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14849-1-PBS

Synonyme

Protein interactor of Torsin-1A, Printor, KLHL14, KIAA1384, Kelch-like protein 14

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  WB analysis of HeLa using 14849-1-AP (same clone as 14849-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 14849-1-AP (same clone as 14849-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 14849-1-AP (same clone as 14849-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 14849-1-AP (same clone as 14849-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse heart using 14849-1-AP (same clone as 14849-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 14849-1-AP (same clone as 14849-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse ovary using 14849-1-AP (same clone as 14849-1-PBS)

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 14849-1-AP (same clone as 14849-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse lung using 14849-1-AP (same clone as 14849-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 14849-1-AP (same clone as 14849-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 14849-1-AP (same clone as 14849-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 14849-1-AP (same clone as 14849-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 14849-1-AP (same clone as 14849-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14849-1-AP (KLHL14-Specific Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 14849-1-AP (same clone as 14849-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14849-1-AP (KLHL14-Specific Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 14849-1-AP (same clone as 14849-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14849-1-AP (KLHL14-Specific Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 14849-1-AP (same clone as 14849-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14849-1-AP (KLHL14-Specific Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 14849-1-AP (same clone as 14849-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using KLHL14-Specific antibody (14849-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14849-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA KLHL14-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: kelch-like 14 (Drosophila)
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 39-45 kDa
• GenBank-Zugangsnummer: NM_020805
• Gene symbol: KLHL14
• Gene ID (NCBI): 57565
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KLHL14, a member of the Kelch-like gene family, has six Kelch domains and is localized to the endoplasmic reticulum. KLHL14, a novel tumor suppressor, is a subunit of a ubiquitin ligase that associates with the endoplasmic reticulum-associated protein degradation (ERAD) machinery, regulating of B cell receptor-dependent NF-κB signaling (PMID: 32127472). KLHL14 has two isoforms of 70kDa and 43kDa.