KLHL18 Polyklonaler Antikörper

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• KD/KO Validated

KLHL18 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF,ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 17229-1-AP

Synonyme

kelch like 18 (Drosophila), Kelch like protein 18, KIAA0795, KLHL18

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 17229-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17229-1-AP (KLHL18 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 17229-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 17229-1-AP (KLHL18 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17229-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17229-1-AP (KLHL18 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 17229-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 17229-1-AP (KLHL18 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17229-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17229-1-AP (KLHL18 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17229-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17229-1-AP (KLHL18 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 17229-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 17229-1-AP (KLHL18 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon using 17229-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 17229-1-AP (KLHL18 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 17229-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17229-1-AP (KLHL18 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 17229-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17229-1-AP (KLHL18 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 17229-1-AP

  Immunofluorescent analysis of Hela cells, using KLHL18 antibody 17229-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, COLO 320-Zellen, HEK-293-Zellen, HeLa-Zellen, HL-60-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

17229-1-AP bindet in WB, IHC, IF,ELISA KLHL18 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KLHL18 fusion protein Ag11070
• Vollständiger Name: kelch-like 18 (Drosophila)
• Berechnetes Molekulargewicht: 509 aa, 56 kDa
• Beobachtetes Molekulargewicht: 63 kDa, 56 kDa
• GenBank-Zugangsnummer: BC032620
• Gene symbol: KLHL18
• Gene ID (NCBI): 23276
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IHC	Cell Biosci; KLHL18 inhibits the proliferation, migration, and invasion of non-small cell lung cancer by inhibiting PI3K/PD-L1 axis activity.; Authors - Xizi Jiang
human	WB	Biol Open; The CUL3-KLHL18 ligase regulates mitotic entry and ubiquitylates Aurora-A.; Authors - Moghe Saili S ; KD Validated