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KLHL2 Polyklonaler Antikörper

KLHL2 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14449-1-PBS

Synonyme

Kelch-like protein 2, Actin-binding protein Mayven, ABP KELCH

Filter:
  • Western Blot (WB) analysis of MCF-7 cells using KLHL2 Polyclonal antibody (14449-1-AP)

    WB analysis of MCF-7 using 14449-1-AP (same clone as 14449-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 14449-1-AP (KLHL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14449-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using KLHL2 Polyclonal antibody (14449-1-AP)

    WB analysis using 14449-1-AP (same clone as 14449-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14449-1-AP (KLHL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14449-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using KLHL2 Polyclonal antibody (14449-1-AP)

    WB analysis of human brain using 14449-1-AP (same clone as 14449-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 14449-1-AP (KLHL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14449-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using KLHL2 Polyclonal antibody (14449-1-AP)

    IF Staining of MCF-7 using 14449-1-AP (same clone as 14449-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using KLHL2 antibody (14449-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 14449-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

14449-1-PBS bindet in WB, IF/ICC, Indirect ELISA KLHL2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen KLHL2 fusion protein Ag5950
Vollständiger Name kelch-like 2, Mayven (Drosophila)
Berechnetes Molekulargewicht 66 kDa
Beobachtetes Molekulargewicht 66 kDa
GenBank-ZugangsnummerBC022503
Gene symbol KLHL2
Gene ID (NCBI) 11275
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KLHL2 contains an SH3-binding domain in the N terminus and a POZ domain and 6 tandem repeats of approximately 50 amino acids each in the C terminus. In astrocytoma/glioblastoma cells, KLHL2 colocalized with actin filaments in the cytoskeleton remodeling regions in stress fibers and in patchy cortical actin-rich regions of the cell margins. In primary rat hippocampal neurons, it was highly expressed in the cell body and in neurite processes(PMID: 10397770).