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KLHL2 Rekombinanter Antikörper

KLHL2 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250148G5

Kat-Nr. : 85893-1-PBS

Synonyme

ABP KELCH, Actin-binding protein Mayven, Kelch-like protein 2

Filter:
  • Western Blot (WB) analysis of various lysates using KLHL2 Recombinant antibody (85893-1-RR)

    WB analysis using 85893-1-RR (same clone as 85893-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85893-1-RR (KLHL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85893-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using KLHL2 Recombinant antibody (85893-1-RR)

    IF Staining of MCF-7 using 85893-1-RR (same clone as 85893-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using KLHL2 antibody (85893-1-RR, Clone: 250148G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85893-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

85893-1-PBS bindet in WB, IF/ICC, Indirect ELISA KLHL2 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen KLHL2 fusion protein Ag5950
Vollständiger Name kelch-like 2, Mayven (Drosophila)
Berechnetes Molekulargewicht 66 kDa
Beobachtetes Molekulargewicht 66 kDa
GenBank-ZugangsnummerBC022503
Gene symbol KLHL2
Gene ID (NCBI) 11275
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KLHL2 contains an SH3-binding domain in the N terminus and a POZ domain and 6 tandem repeats of approximately 50 amino acids each in the C terminus. In astrocytoma/glioblastoma cells, KLHL2 colocalized with actin filaments in the cytoskeleton remodeling regions in stress fibers and in patchy cortical actin-rich regions of the cell margins. In primary rat hippocampal neurons, it was highly expressed in the cell body and in neurite processes(PMID: 10397770).