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KLHL32 Polyklonaler Antikörper

KLHL32 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28621-1-PBS

Synonyme

RP1 39B17.1, KIAA1900, Kelch-like protein 32, Kelch like protein 32, BTB and kelch domain-containing protein 5

Filter:
  • Western Blot (WB) analysis of various lysates using KLHL32 Polyclonal antibody (28621-1-AP)

    WB analysis using 28621-1-AP (same clone as 28621-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28621-1-AP (KLHL32 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28621-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using KLHL32 Polyclonal antibody (28621-1-AP)

    IF Staining of U2OS using 28621-1-AP (same clone as 28621-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using KLHL32 antibody (28621-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (red). This data was developed using the same antibody clone with 28621-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

28621-1-PBS bindet in WB, IF/ICC, Indirect ELISA KLHL32 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen KLHL32 fusion protein Ag29003
Vollständiger Name kelch-like 32 (Drosophila)
Berechnetes Molekulargewicht 470 aa, 53 kDa
Beobachtetes Molekulargewicht70 kDa
GenBank-ZugangsnummerBC071627
Gene symbol KLHL32
Gene ID (NCBI) 114792
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KLHL32 (Kelch-like protein 32), also known BKLHD5, which is located in the cytoplasm. The expression level is high in the central nervous system, especially in astrocytes. In addition, it is also expressed in the motor cilia of fallopian tube. But the overall expression abundance is low. KLHL32 is mainly involved in the intracellular ubiquitination process. Ubiquitin is an important cellular regulatory mechanism, through which protein can be labeled to degrade or change its function. KLHL32 interacts with other protein through its BTB domain and Kelch repeat domain to help regulate this process. It is also a prognostic marker in glioblastoma and renal clear cell carcinoma. The molecular weight of KLHL32 is 70 kDa.