KLK11 Monoklonaler Antikörper

KLK11 Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

1E2B4

Kat-Nr. : 67427-1-PBS

Synonyme

Kallikrein 11, 1E2B4, EC:3.4.21.-, Kallikrein11, Kallikrein-11

---

• 

  WB analysis using 67427-1-Ig (same clone as 67427-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67427-1-Ig (KLK11 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67427-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67427-1-Ig (same clone as 67427-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67427-1-Ig (KLK11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67427-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67427-1-Ig (same clone as 67427-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67427-1-Ig (KLK11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67427-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67427-1-Ig (same clone as 67427-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67427-1-Ig (KLK11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67427-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67427-1-Ig (same clone as 67427-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67427-1-Ig (KLK11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67427-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 67427-1-Ig (same clone as 67427-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67427-1-Ig (KLK11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67427-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 67427-1-Ig (same clone as 67427-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67427-1-Ig (KLK11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67427-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human breast cancer using 67427-1-Ig (same clone as 67427-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using KLK11 antibody (67427-1-Ig, Clone: 1E2B4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67427-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human breast cancer using 67427-1-Ig (same clone as 67427-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using KLK11 antibody (67427-1-Ig, Clone: 1E2B4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67427-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

67427-1-PBS bindet in WB, IHC, IF-P, ELISA KLK11 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: KLK11 fusion protein Ag29895
• Vollständiger Name: kallikrein-related peptidase 11
• Berechnetes Molekulargewicht: 250 aa, 27 kDa
• Beobachtetes Molekulargewicht: 30-40 kDa
• GenBank-Zugangsnummer: BC022068
• Gene symbol: KLK11
• Gene ID (NCBI): 11012
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KLK11(Kallikrein-11) is also named as PRSS20, TLSP and belongs to the peptidase S1 family and Kallikrein subfamily. It is a multifunctinal protease cleaving several substrates for kallikrein and trypsin and involved in normal physiology processes in bronchus. This full length protein has a signal peptide, a propeptide and four glycosylation sites. It has 4 isoforms produced by alternative splicing with the molecular weight of 31 kDa, 27 kDa,34 kDa and 30 kDa.