KLK3/PSA Monoklonaler Antikörper

KLK3/PSA Monoklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

CloneNo.

4A1E2

Kat-Nr. : 60338-1-Ig

Synonyme

APS, Gamma seminoprotein, hK3, Kallikrein 3, kallikrein related peptidase 3, KLK2A1, KLK3, KLK3,PSA, P 30 antigen, Prostate specific antigen, PSA, Semenogelase, Seminin

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Galerie der Validierungsdaten

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  WB analysis using 60338-1-Ig

  PC-3(PSA-), DU 145(PSA-) and LNCaP (PSA+) cell lysates were subjected to SDS PAGE followed by western blot with 60338-1-Ig (KLK3/PSA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 60338-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60338-1-Ig (KLK3/PSA antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 60338-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60338-1-Ig (KLK3/PSA antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 60338-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using (KLK3,PSA Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 60338-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using (KLK3,PSA Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 60338-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60338-1-Ig (KLK3,PSA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 60338-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60338-1-Ig (KLK3,PSA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:2500-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below

Produktinformation

60338-1-Ig bindet in WB, IHC, ELISA KLK3/PSA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: KLK3/PSA fusion protein Ag1060
• Vollständiger Name: kallikrein-related peptidase 3
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 30-34 kDa
• GenBank-Zugangsnummer: BC005307
• Gene symbol: KLK3
• Gene ID (NCBI): 354
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. kallikrein-related peptidase 3, which is also called PSA (prostate-specific antigen) in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. KLK3 has 5 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB	Cell Death Dis; N-glycosylation of GDF15 abolishes its inhibitory effect on EGFR in AR inhibitor-resistant prostate cancer cells; Authors - Rong Wang
human	WB	J Cell Commun Signal; Estrogens drive the endoplasmic reticulum-associated degradation and promote proto-oncogene c-Myc expression in prostate cancer cells by androgen receptor/estrogen receptor signaling; Authors - Yalcin Erzurumlu
human	WB	Cell Signal; 1,25(OH)2 D3 induced vitamin D receptor signaling negatively regulates endoplasmic reticulum-associated degradation (ERAD) and androgen receptor signaling in human prostate cancer cells; Authors - Yalcin Erzurumlu
human	WB	J Nat Med; Potent carotenoid astaxanthin expands the anti-cancer activity of cisplatin in human prostate cancer cells; Authors - Yalcin Erzurumlu
human	WB	Cell Signal; Triiodothyronine positively regulates endoplasmic reticulum-associated degradation (ERAD) and promotes androgenic signaling in androgen-dependent prostate cancer cells; Authors - Yalcin Erzurumlu
human	WB	ACS Appl Mater Interfaces; Two-in-One Polymeric NO-Donor Prodrugs Mediate Precision and Synergistic Prostate Cancer Treatment; Authors - Bingbing Zhao