KLK3/PSA Rekombinanter Antikörper

KLK3/PSA Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

241263F8

Kat-Nr. : 84059-5-RR

Synonyme

KLK3, 241263F8, APS, EC:3.4.21.77, Gamma-seminoprotein

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  WB analysis of LNCaP using 84059-5-RR

  LNCaP cells were subjected to SDS PAGE followed by western blot with 84059-5-RR (KLK3/PSA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 84059-5-RR

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 84059-5-RR (KLK3/PSA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of LNCaP using 84059-5-RR

  Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using KLK3/PSA antibody (84059-5-RR, Clone: 241263F8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HeLa using 84059-5-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug KLK3/PSA Recombinant antibody (84059-5-RR, Clone:241263F8) and PE-conjugated Goat Anti-Rabbit IgG(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 84059-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84059-5-RR against Human KLK3/PSA were performed. The affinity constant is 149 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: LNCaP-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84059-5-RR bindet in WB, IHC, IF/ICC, FC (Intra), ELISA KLK3/PSA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: KLK3/PSA fusion protein Eg0869
• Vollständiger Name: kallikrein-related peptidase 3
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 30-34 kDa
• GenBank-Zugangsnummer: NM_001648.2
• Gene symbol: KLK3
• Gene ID (NCBI): 354
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. kallikrein-related peptidase 3, which is also called PSA (prostate-specific antigen) in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. KLK3 has 5 isoforms  produced by alternative splicing.