KLK8 Monoklonaler Antikörper

KLK8 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2F3A8

Kat-Nr. : 68247-1-PBS

Synonyme

Kallikrein 8, Kallikrein-8, Kallikrein8, kallikrein related peptidase 8, EC:3.4.21.118

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  WB analysis using 68247-1-Ig (same clone as 68247-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68247-1-Ig (KLK8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68247-1-PBS in a different storage buffer formulation.

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  WB analysis of EC109 using 68247-1-Ig (same clone as 68247-1-PBS)

  EC109 cells were subjected to SDS PAGE followed by western blot with 68247-1-Ig (KLK8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68247-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 68247-1-Ig (same clone as 68247-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 68247-1-Ig (KLK8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68247-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 68247-1-Ig (same clone as 68247-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68247-1-Ig (KLK8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68247-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 68247-1-Ig (same clone as 68247-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68247-1-Ig (KLK8 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68247-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68247-1-Ig (same clone as 68247-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68247-1-Ig (KLK8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68247-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68247-1-Ig (same clone as 68247-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68247-1-Ig (KLK8 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68247-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 68247-1-Ig (same clone as 68247-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using KLK8 antibody (68247-1-Ig, Clone: 2F3A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68247-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 68247-1-Ig (same clone as 68247-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using KLK8 antibody (68247-1-Ig, Clone: 2F3A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68247-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68247-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA KLK8 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: KLK8 fusion protein Ag5642
• Vollständiger Name: kallikrein-related peptidase 8
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC040887
• Gene symbol: KLK8
• Gene ID (NCBI): 11202
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KLK8(Kallikrein-8) is also named as NRPN, PRSS19, TADG14.It is a new member of the human kallikrein family, which is predicted to be secreted and expected to be present in biological fluids (PMID:12507964).The protein is detectable in ovarian cancer tissue extracts, serum, and ascites fluid, indicating that it may serve as a new ovarian cancer marker (PMID:12782581). KLK8 has one predicted glycosylation site (PMID:20940292) and it has some isoforms produced by alternative splicing.