KLRB1/CD161 Rekombinanter Antikörper

KLRB1/CD161 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Ratte

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

241840F3

Kat-Nr. : 98249-1-RR

Synonyme

Klrb1a, Killer cell lectin-like receptor subfamily B member 1A, CD161a, CD161 antigen-like family member A, Antigen 3.2.3

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  FC experiment of rat splenocytes using 98249-1-RR

  1x10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat KLRB1/CD161 Rabbit Recombinant Antibody (98249-1-RR, Clone: 241840F3) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  FC experiment of rat splenocytes using 98249-1-RR

  1x10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat KLRB1/CD161 Rabbit Recombinant Antibody (98249-1-RR, Clone:241840F3)(red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98249-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98249-1-RR against Rat KLRB1/CD161 were performed. The affinity constant is 88.8 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Ratten-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98249-1-RR bindet in FC KLRB1/CD161 und zeigt Reaktivität mit Ratten

• Getestete Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: killer cell lectin-like receptor subfamily B, member 1A
• Berechnetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: NM_001010964.1
• Gene symbol: Klrb1a
• Gene ID (NCBI): 362443
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

CD161, also known as KLRB1 or NKR-P1A, is a type II transmembrane C-type lectin-like receptor and is expressed on the cell membrane as disulfide-linked homodimer (PMID: 8077657). It is expressed by the majority of NK cells and subsets of peripheral T cells, including both CD4+ and CD8+ T cells, and is expressed preferentially on adult T cells with a "memory" antigenic phenotype (PMID: 8077657; 22566826). Expression of CD161 correlates with the cytotoxic function of CD16+ NK cells, and ligation of CD161 with its ligand LLT1 inhibits NK cell cytotoxicity and cytokine secretion (PMID: 29686665).