KMO Polyklonaler Antikörper

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• KD/KO Validated

KMO Polyklonal Antikörper für IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

23

Rezensionen (1)

Kat-Nr. : 10698-1-AP

Synonyme

KMO, Kynurenine 3 hydroxylase, Kynurenine 3 monooxygenase

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 10698-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10698-1-AP (KMO antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10698-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10698-1-AP (KMO antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 10698-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10698-1-AP (KMO antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 10698-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10698-1-AP (KMO antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 10698-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 10698-1-AP (KMO Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 10698-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10698-1-AP (KMO Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 10698-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10698-1-AP (KMO Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human breast cancer using 10698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10698-1-AP (KMO antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 10698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10698-1-AP (KMO antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse heart using 10698-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10698-1-AP (KMO Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse heart using 10698-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10698-1-AP (KMO Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human breast cancer using 10698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10698-1-AP (KMO antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 10698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10698-1-AP (KMO antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 10698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10698-1-AP (KMO antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 10698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10698-1-AP (KMO antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 10698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10698-1-AP (KMO antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 10698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10698-1-AP (KMO antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 10698-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10698-1-AP (KMO antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HepG2 using 10698-1-AP

  Immunofluorescent analysis of HepG2 cells using 10698-1-AP (KMO antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 10698-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using KMO antibody (10698-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of MCF-7 using 10698-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using KMO antibody (10698-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of MCF-7 using 10698-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 10698-1-AP (KMO antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Mammakarzinomgewebe, humanes Prostatakarzinomgewebe, Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 14 publications below
• IHC: See 8 publications below
• IF: See 9 publications below

Produktinformation

10698-1-AP bindet in WB, IHC, IF, ELISA KMO und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KMO fusion protein Ag1109
• Vollständiger Name: kynurenine 3-monooxygenase (kynurenine 3-hydroxylase)
• Berechnetes Molekulargewicht: 486 aa, 56 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC005297
• Gene symbol: KMO
• Gene ID (NCBI): 8564
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KMO(Kynurenine 3-monooxygenase) is an NADPH-dependent flavin monooxygenase, catalysing the hydroxylation of the L-kynurenine to form L-3-hydroxykynurenine. KMO is a membrane protein located on the outer membrane of mitochondria. Tissue distribution studies have revealed that, in rats, highest enzyme activity is found in kidney and liver, with brain having the least activity in comparison to peripheral organs(PMID: 9237672).

Publikationen

Species	Application	Title
mouse	IF	J Hazard Mater; Particulate matter-induced metabolic recoding of epigenetics in macrophages drives pathogenesis of chronic obstructive pulmonary disease; Authors - Myungkyung Noh
human	WB	Circ Res; Activation of NAD(P)H Oxidase by Tryptophan-Derived 3-Hydroxykynurenine Accelerates Endothelial Apoptosis and Dysfunction In Vivo.; Authors - Qiongxin Wang
human, mouse	IF	J Am Soc Nephrol; Loss of Kynurenine 3-Mono-oxygenase Causes Proteinuria.; Authors - Ron Korstanje
human	IHC	J Intern Med; Evidence that a deviation in the kynurenine pathway aggravates atherosclerotic disease in humans.; Authors - R Baumgartner
human	WB	Mol Oncol; Cancer-associated fibroblasts educate normal fibroblasts to facilitate cancer cell spreading and T cell suppression.; Authors - Go Itoh
human	WB	PLoS Genet; A novel role for kynurenine 3-monooxygenase in mitochondrial dynamics.; Authors - Daniel C Maddison

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hala (Verified Customer) (03-23-2022)

works good

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Huh6 and Hepg2

KMO Antibody Western Blot validation (1/1000 dilution) in Huh6 and Hepg2 (Cat no:10698-1-AP)

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