KMT2C Rekombinanter Antikörper

KMT2C Rekombinant Antikörper für IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240542A2

Kat-Nr. : 83595-4-PBS

Synonyme

MLL3, 240542A2, HALR, Homologous to ALR protein, KIAA1506

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  IF Staining of MCF-7 using 83595-4-RR (same clone as 83595-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using KMT2C antibody (83595-4-RR, Clone: 240542A2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (green). This data was developed using the same antibody clone with 83595-4-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 83595-4-RR (same clone as 83595-4-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug KMT2C Recombinant antibody (83595-4-RR, Clone:240542A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83595-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83595-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83595-4-RR against Human KMT2C were performed. The affinity constant is 2.67 nM.

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Geprüfte Anwendungen

Produktinformation

83595-4-PBS bindet in IF/ICC, FC (Intra), ELISA KMT2C und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: KMT2C fusion protein Ag28875
• Vollständiger Name: myeloid/lymphoid or mixed-lineage leukemia 3
• Berechnetes Molekulargewicht: 541 kDa
• GenBank-Zugangsnummer: NM_170606
• Gene symbol: KMT2C
• Gene ID (NCBI): 58508
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lysine (K)-specific methyltransferase 2C (KMT2C, also known as MLL3) belongs to the mixed-lineage leukemia (MLL) family of histone methyltransferases which methylate the histone 3 tail at lysine 4 (H3K4) as part of the complex proteins associated with Set 1 (COMPASS) complex. Although originally identified as oncogenic fusions in leukemia, genome-wide mutation studies have revealed frequent, presumably loss-of-function, mutations in various members of the MLL family, including MLL2/KMT2B, MLL3/KMT2C, and MLL4/KMT2D in a variety of malignancies, particularly solid tumors. Mechanistic studies of KMT2C in normal cells have focused primarily on its role in enhancer regulation by deposition of H3K4me1 marks.