Kininogen 1 Polyklonaler Antikörper

Kininogen 1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 11926-1-AP

Synonyme

KNG1, Ile Ser Bradykinin Bradykinin, HMWK, Fitzgerald factor, Bradykinin

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  WB analysis of HeLa using 11926-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11926-1-AP (Kininogen 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11926-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11926-1-AP (Kininogen 1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A2780 using 11926-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 11926-1-AP (Kininogen 1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11926-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11926-1-AP (Kininogen 1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11926-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11926-1-AP (Kininogen 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 11926-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 11926-1-AP (Kininogen 1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 11926-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11926-1-AP (Kininogen 1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 11926-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11926-1-AP (Kininogen 1 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 11926-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11926-1-AP (Kininogen 1 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human endometrial cancer using 11926-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 11926-1-AP (Kininogen 1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human endometrial cancer using 11926-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 11926-1-AP (Kininogen 1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 11926-1-AP

  Immunofluorescent analysis of HepG2 cells, using KNG1 antibody 11926-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A2780-Zellen, COLO 320-Zellen, MCF-7-Zellen, Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Endometriumkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

11926-1-AP bindet in WB, IHC, IF/ICC, CoIP, ELISA Kininogen 1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Kininogen 1 fusion protein Ag2574
• Vollständiger Name: kininogen 1
• Berechnetes Molekulargewicht: 644 aa, 72 kDa
• Beobachtetes Molekulargewicht: 48 kDa, 72 kDa
• GenBank-Zugangsnummer: BC060039
• Gene symbol: Kininogen 1
• Gene ID (NCBI): 3827
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Kininogens are inhibitors of thiol proteases. Kininogen 1 plays important role in Kinin-kallikrein system. This KNG1 gene contains 10 exons and encodes two proteins through alternative splicing: high molecular weight kininogen (HMWK) (72kDa) and low molecular weight kininogen (LMWK) (48kDa) (PMID: 37442062). HMWK is produced by the liver together with prekallikrein. It acts mainly as a cofactor on coagulation and inflammation, and has no intrinsic catalytic activity. LMWK is produced locally by numerous tissues, and secreted together with tissue kallikrein. 11926-1-AP was generated against N-terminal 300 aa of HMW kininogen. It can bind both HMW and LMW kiniogen.

Publikationen

Species	Application	Title
human	WB,CoIP	EBioMedicine; An HBV susceptibility variant of KNG1 modulates the therapeutic effects of interferons α and λ1 in HBV infection by promoting MAVS lysosomal degradation; Authors - Bin Zhang
human	WB	Cell Physiol Biochem; Novel Serum Biomarkers Detected by Protein Array in Polycystic Ovary Syndrome with Low Progesterone Level.; Authors - Qin Zheng
rat	WB	PLoS One; Proteomic analysis of rat serum revealed the effects of chronic sleep deprivation on metabolic, cardiovascular and nervous system.; Authors - Bo Ma
rat	WB	Biol Pharm Bull; Proteomic analysis reveals the renoprotective effect of Tribulus terrestris against obesity-related glomerulopathy in rats.; Authors - Yue-Hua Jiang
mouse	WB	Med Sci Monit; Isobaric Tagging for Relative and Absolute Protein Quantification (iTRAQ)-Based Quantitative Proteomics Analysis of Differentially Expressed Proteins 1 Week After Spinal Cord Injury in a Rat Model.; Authors - Shen Liu
rat	WB,IF	JID Innov; Kininogen-Nitric Oxide Signaling at Nearby Nonexcited Acupoints after Long-Term Stimulation.; Authors - Ting Wang