KPNA3 Polyklonaler Antikörper

KPNA3 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28050-1-PBS

Synonyme

Importin alpha Q2, Importin subunit alpha-4, Karyopherin subunit alpha 3, Karyopherin subunit alpha-3, Qip2

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  WB analysis using 28050-1-AP (same clone as 28050-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28050-1-AP (KPNA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28050-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 28050-1-AP (same clone as 28050-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28050-1-AP (KPNA3 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28050-1-PBS in a different storage buffer formulation.

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  IHC staining of human brown disease using 28050-1-AP (same clone as 28050-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 28050-1-AP (KPNA3 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28050-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 28050-1-AP (same clone as 28050-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using KPNA3 antibody (28050-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green). This data was developed using the same antibody clone with 28050-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

28050-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA KPNA3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KPNA3 fusion protein Ag27755
• Vollständiger Name: karyopherin alpha 3 (importin alpha 4)
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 57-60 kDa
• GenBank-Zugangsnummer: BC017355
• Gene symbol: KPNA3
• Gene ID (NCBI): 3839
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KPNA3, karyopherin subunit alpha 3, also known as SRP1 or IPOA4. It is expected to be located in cytoplasm and nucleus, the protein is ubiquitous and highly expressed in heart, skeletal muscle. The calculated molecular weight of KPNA3 is 57 kDa. Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin.