KPNA4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

KPNA4 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 12463-1-AP

Synonyme

Importin alpha Q1, Importin subunit alpha 4, IPOA3, Karyopherin subunit alpha 4, KPNA4, QIP1, SRP3

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Galerie der Validierungsdaten

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  WB analysis using 12463-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12463-1-AP (KPNA4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12463-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12463-1-AP (KPNA4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 12463-1-AP

  IP Result of anti-KPNA4 (IP:12463-1-AP, 4ug; Detection:12463-1-AP 1:600) with A549 cells lysate 2000ug.

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  IHC staining of human colon cancer using 12463-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12463-1-AP (KPNA4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 12463-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 12463-1-AP (KPNA4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, Jurkat-Zellen, Maushodengewebe, NIH/3T3-Zellen, Rattenhodengewebe
• Erfolgreiche IP: A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• CoIP: See 1 publications below

Produktinformation

12463-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA KPNA4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KPNA4 fusion protein Ag3133
• Vollständiger Name: karyopherin alpha 4 (importin alpha 3)
• Berechnetes Molekulargewicht: 521 aa, 58 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC034493
• Gene symbol: KPNA4
• Gene ID (NCBI): 3840
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KPNA4, also named importin subunit alpha-3, karyopherin-alpha4, is a cytoplasmic protein that recognizes nuclear localization signals (NLSs) and dock NLS-containing proteins to the nuclear pore complex. Nuclear import, mediated in part by karyopherin-α (KPNA)/importin-α subtypes, regulates transcription factor access to the genome and determines cell fate. KPNA4-mediated nuclear transport of Ras-responsive element-binding protein (RREB1), which sustains Ras/ERK pathway signaling through repressing miR-143/145 expression (PMID: 31822798). KPNA4 is one of the main isoforms that is activated in many human cancers. KPNA4 expression was elevated in head and neck of squamous cell carcinoma (PMID: 33188837).

Publikationen

Species	Application	Title
human	WB,CoIP	Virol Sin; African Swine Fever Virus MGF360-12L Inhibits Type I Interferon Production by Blocking the Interaction of Importin α and NF-κB Signaling Pathway.; Authors - Yisha Zhuo
human	WB	Oncogene; The Wnt/β-catenin/VASP positive feedback loop drives cell proliferation and migration in breast cancer.; Authors - Kai Li ; KD Validated
mouse	WB	J Neurosci; The subcellular distribution of HDAC1 in neurotoxic conditions is dependent on serine phosphorylation.; Authors - Yunjiao Zhu
mouse	WB	Exp Gerontol; Age-dependent changes in nuclear-cytoplasmic signaling in skeletal muscle.; Authors - Shama R Iyer
human	WB	Tumour Biol; Reanalysis of microRNA expression profiles identifies novel biomarkers for hepatocellular carcinoma prognosis.; Authors - Zhengqiang Wang
human	WB	Viruses; Ivermectin Inhibits HBV Entry into the Nucleus by Suppressing KPNA2; Authors - Anna Nakanishi