KPNA4 Rekombinanter Antikörper

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• KD/KO Validated

KPNA4 Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

230098H3

Kat-Nr. : 82898-1-RR

Synonyme

230098H3, Importin subunit alpha-3, IPOA3, Karyopherin subunit alpha-4, KPNA 4

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  WB analysis using 82898-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82898-1-RR (KPNA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 82898-1-RR

  WB result of KPNA4 antibody (82898-1-RR; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KPNA4 transfected A549 cells.

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  IF Staining of U2OS using 82898-1-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using KPNA4 antibody (82898-1-RR, Clone: 230098H3 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HeLa using 82898-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.13 ug Anti-Human KPNA4 (82898-1-RR, Clone:230098H3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.13 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82898-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82898-1-RR against Human KPNA4 were performed. The affinity constant is 0.417 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82898-1-RR bindet in WB, IF/ICC, FC (Intra), ELISA KPNA4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: KPNA4 fusion protein Ag3133
• Vollständiger Name: karyopherin alpha 4 (importin alpha 3)
• Berechnetes Molekulargewicht: 521 aa, 58 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC034493
• Gene symbol: KPNA4
• Gene ID (NCBI): 3840
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.