KPTN Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

KPTN Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Kat-Nr. : 16094-1-AP

Synonyme

KICSTOR complex protein kaptin, Kaptin, Actin-associated protein 2E4

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  WB analysis using 16094-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16094-1-AP (KPTN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16094-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16094-1-AP (KPTN antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 16094-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16094-1-AP (KPTN antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human cerebellum using 16094-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 16094-1-AP (KPTN antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 16094-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16094-1-AP (KPTN antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16094-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16094-1-AP (KPTN antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 16094-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 16094-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 16094-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 16094-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 16094-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 16094-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 16094-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 16094-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 16094-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 16094-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 16094-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 16094-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 16094-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 16094-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 16094-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 16094-1-AP (KPTN antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Cerebellum-Gewebe, Maus-Skelettmuskelgewebe, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Herzgewebe, humanes Lungengewebe, humanes Eierstockgewebe, humanes Plazenta-Gewebe, humanes Hautgewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 12 publications below

Produktinformation

16094-1-AP bindet in WB, IHC, IF/ICC, ELISA KPTN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KPTN fusion protein Ag8785
• Vollständiger Name: kaptin (actin binding protein)
• Berechnetes Molekulargewicht: 436 aa, 48 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC009249
• Gene symbol: KPTN
• Gene ID (NCBI): 11133
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Kaptin, also named as KPTN and Actin-associated protein 2E4, originally isolated from human blood platelets and likely to be involved in the actin rearrangements occurring during activation. It plays a unique role in the actin rearrangements that accompany platelet activation and stereocilia formation. Kaptin is necessary for normal neuromorphogenesis. It may play a role in producing the sensory apparatus in hair cells.

Publikationen

Species	Application	Title
human	WB	Nature; Structure of the nutrient-sensing hub GATOR2.; Authors - Max L Valenstein
human	WB	Nature; Architecture of the human GATOR1 and GATOR1-Rag GTPases complexes.; Authors - Kuang Shen
human	WB	Nature; KICSTOR recruits GATOR1 to the lysosome and is necessary for nutrients to regulate mTORC1.; Authors - Rachel L Wolfson ; KD Validated
human	WB	Proc Natl Acad Sci U S A; Rag-Ragulator is the central organizer of the physical architecture of the mTORC1 nutrient-sensing pathway; Authors - Max L Valenstein
human	WB	Cell Rep; CDK5-PRMT1-WDR24 signaling cascade promotes mTORC1 signaling and tumor growth; Authors - Shasha Yin ; KO Validated
human	WB	Cells; The SZT2 Interactome Unravels New Functions of the KICSTOR Complex.; Authors - Cecilia Cattelani