Cytokeratin 1-specific Rekombinanter Antikörper

Cytokeratin 1-specific Rekombinant Antikörper für WB, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230261F9

Kat-Nr. : 82972-1-PBS

Synonyme

KRT1-specific, Cytokeratin 1, 230261F9, CK-1, Cytokeratin,Keratin

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  WB analysis using 82972-1-RR (same clone as 82972-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82972-1-RR (KRT1-specific antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82972-1-PBS in a different storage buffer formulation.

• 

  FC experiment of A431 using 82972-1-RR (same clone as 82972-1-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human KRT1-specific (82972-1-RR, Clone:230261F9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82972-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

82972-1-PBS bindet in WB, FC (Intra), Indirect ELISA Cytokeratin 1-specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: keratin 1
• Berechnetes Molekulargewicht: 66 kDa
• Beobachtetes Molekulargewicht: 67 kDa
• GenBank-Zugangsnummer: NM_006121
• Gene symbol: KRT1
• Gene ID (NCBI): 3848
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin-1 is type II keratin. It is a specific marker for terminal differentiation in mammalian epidermis. It is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma.