Cytokeratin 13 Monoklonaler Antikörper

Cytokeratin 13 Monoklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1E10G2

Kat-Nr. : 66684-1-Ig

Synonyme

CK 13, CK13, Cytokeratin 13, K13, keratin 13, KRT13

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Galerie der Validierungsdaten

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  WB analysis of A431 using 66684-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66684-1-Ig (KRT13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skin using 66684-1-Ig

  rat skin tissue were subjected to SDS PAGE followed by western blot with 66684-1-Ig (KRT13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 66684-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66684-1-Ig (KRT13 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 66684-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66684-1-Ig (KRT13 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 66684-1-Ig

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66684-1-Ig (Cytokeratin 13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 66684-1-Ig

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66684-1-Ig (Cytokeratin 13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 66684-1-Ig

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66684-1-Ig (Cytokeratin 13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 66684-1-Ig

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66684-1-Ig (Cytokeratin 13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66684-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66684-1-Ig (Cytokeratin 13 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66684-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66684-1-Ig (Cytokeratin 13 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human cervical cancer using 66684-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human cervical cancer tissue using Cytokeratin 13 antibody (66684-1-Ig, Clone: 1E10G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of A431 using 66684-1-Ig

  1X10^6 A431 cells were intracellularly stained with 0.2 ug Anti-Human Cytokeratin 13 (66684-1-Ig, Clone:1E10G2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, Rattenhautgewebe
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Ösophaguskarzinomgewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Zervixkarzinomgewebe
• Erfolgreiche Detektion in FC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66684-1-Ig bindet in WB, IHC, IF, FC, ELISA Cytokeratin 13 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 13 fusion protein Ag0217
• Vollständiger Name: keratin 13
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC002661
• Gene symbol: KRT13
• Gene ID (NCBI): 3860
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. The Keratin 13 (KRT13) gene encodes a type I acidic keratin which is expressed in the differentiated cells of non-cornified stratified squamous epithelia. This antibody can react with Cytokeratin 15.